NEET Biomolecules Important Questions: Check Free PDF Download

The topic of Biomolecules is an integral part of the NEET Biology section. Important questions have featured carbohydrates, proteins, enzymes, nucleic acids, lipids, and more. The NEET 2025 exam requires a steady grasp of biochemical concepts and the ability to analyse molecular structures.

This article provides an overview of question patterns categorised according to NEET chapter-wise weightage and contains sample questions of conceptual and numerical kinds. It is based on the frequency, difficulty level, and variations of NEET Biomolecules important questions.

NEET Biomolecules Important Questions

Based on our analysis of the past five years of NEET previous year question paper, below mentioned are the questions on Biomolecules that frequently tested specific core concepts, such as:

Topic	Key Concepts
Carbohydrates	Monosaccharides, disaccharides, polysaccharides, and their biological roles.
Proteins	Amino acids, peptide bonds, primary to quaternary structures, and functions.
Enzymes	Mechanism of enzyme action, factors affecting enzyme activity, and cofactors.
Nucleic Acids	DNA and RNA structure, replication, transcription, and translation.
Lipids	Classification, structure, and functions of fats, oils, and phospholipids.
Miscellaneous	Vitamins, hormones, and their roles in metabolic processes.

Also Read: Important Diagrams For Class 11 Botany for NEET Biology

NEET Biomolecules Important Questions Pattern Analysis

Our analysis of question patterns over the last five years reveals that the NEET exam favours questions that balance theoretical knowledge with practical application. Here is a table representing question trends usually observed in NEET 2025.

Question Type	Description	Example
Direct Theoretical	Requires recalling definitions, structures, and functions.	What is the structure of glucose? or What is the role of ATP in metabolism?
Semi-Analytical	Involves interpreting diagrams or experimental data.	Analyze the DNA double helix diagram and identify the base pairs.
Application-Based	Tests the ability to apply Biomolecules knowledge to other biology topics.	Explain the role of enzymes in digestion. or How do mutations affect protein synthesis?
Comparative	Asks you to compare similar concepts.	Compare DNA and RNA. or Differentiate between saturated and unsaturated fats.

Also Read: NEET Biology Chapter Wise Weightage 2025 - Download PDF

NEET Biomolecules Topic-wise Breakdown

Here’s a detailed categorisation of biomolecules topics that frequently appear in NEET:

Carbohydrates

• Classification: Monosaccharides, disaccharides, and polysaccharides.
• Examples: Glucose, sucrose, starch, and cellulose.
• Functions: Energy storage (glycogen) and structural support (cellulose).

Proteins

• Structure: Amino acids, peptide bonds, and protein folding.
• Functions: Enzymes, antibodies, and structural proteins (e.g., keratin).
• Denaturation: Causes and effects on protein function.

Enzymes

• Mechanism: Lock-and-key model and induced-fit hypothesis.
• Factors: Temperature, pH, and substrate concentration.
• Inhibition: Competitive vs. non-competitive inhibition.

Nucleic Acids

• DNA: Double helix structure, replication, and base pairing.
• RNA: Types (mRNA, tRNA, rRNA) and their roles in protein synthesis.
• Central Dogma: DNA → RNA → Protein.

Lipids

• Types: Fats, oils, phospholipids, and steroids.
• Functions: Energy storage, cell membrane structure, and signaling.

Vitamins and Hormones

• Vitamins: Water-soluble (B, C) vs. fat-soluble (A, D, E, K).
• Hormones: Insulin, glucagon, and their roles in metabolism.

Also Read: NEET Biology Question Paper 2024, 2023, 2022, 2021

Important Formulas and Definitions

1. Glycosidic Bond: A covalent bond that joins a carbohydrate molecule to another group
2. Peptide Bond: A covalent bond formed between amino acids
3. Michaelis-Menten Equation: Determines the rate of enzymatic reactions
4. Central Dogma: The flow of genetic information from DNA to RNA to protein

Key Pointers for Answering NEET Biomolecules Questions

1. Memorise the structures and functions of key biomolecules.
2. Practice interpreting diagrams, such as DNA replication or enzyme-substrate interactions.
3. Focus on high-weightage topics like enzymes, nucleic acids, and carbohydrates.
4. Use NEET previous year question papers to identify frequently tested areas.

Solved Examples

Example 1: Conceptual Problem
Problem: Which of the following is a disaccharide?
Option a) Glucose
Option b) Sucrose
Option c) Starch
Option d) Cellulose

Solution: The correct answer is b) Sucrose, as it is composed of glucose and fructose.

Example 2: Calculation-Based Problem
Problem: If a DNA strand has 30% adenine, what would be the percentage of guanine it has?

a) 10%
b) 20%
c) 30%
d) 40%

Solution: The correct answer is b) 20%. 

According to Chargaff’s rule, in DNA, the amount of adenine (A )= thymine (T), and the amount of cytosine C = guanine (G). Therefore, if adenine (A) = 30%, then thymine (T) = 30%. And since the remaining 40% is equally divided between cytosine (C) and guanine (G);

Thus, the answer is G = 20%.G = 0

NEET Biomolecules Important Questions: High Weightage Topics & Question Types

NEET Biomolecules Important Questions: High Weightage Topics & Question Types

High-Weightage Topic	Types of Questions Asked	Example Question
Carbohydrates	Classification and functions	Which of the following is a reducing sugar?
Proteins	Structure and denaturation	What is the primary structure of a protein?
Enzymes	Mechanism and inhibition	Which factor does NOT affect enzyme activity?
Nucleic Acids	DNA replication and transcription	What is the role of RNA polymerase in transcription?
Lipids	Classification and functions	Which lipid is a major component of cell membranes?

Focusing on NEET Biomolecules important questions, covering all important elements such as carbohydrates, proteins, enzymes, nucleic acids, and lipids and practising past papers and NEET mock tests regularly, with a mix of conceptual and numerical problems, along with a thorough understanding of molecular structures and biochemical processes, will ultimately pave the way to your brilliant performance in the NEET exam.

Also Read: NEET Biology Syllabus